沈阳农业大学

百合品质调控与种质创新研究团队

团队概况

沈阳农业大学百合品质调控与种质创新研究团队所属研究平台为设施园艺省部共建教育部重点实验室、北方园艺设施设计与应用技术国家地方联合工程研究中心。从事百合研究20余年。承担省部级以上研究课题30余项，其中国家自然科学基金8项，国家重点研发课题1项。获辽宁省科技进步二等奖2项，省自然科学学术成果一等奖2项、二等奖3项；获得和申请国家发明专利15项、实用新型专利9项；发表学术论文200余篇，主编著作6部，参编著作20余部。团队现有教授2人、副教授1人、讲师 3人，博士生11人，硕士生19人。孙红梅教授，团队带头人，博士生导师，主要从事百合种球品质调控与发育休眠机理研究；孙晓梅教授，博士生导师，主要从事百合繁殖与杂交育种工作；陈丽静副教授，博士生导师，主要从事百合抗寒机理与种质创新研究；王锦霞讲师，主要从事百合设施高效栽培生理研究；王春夏讲师，主要从事百合资源收集与评价研究；王志平讲师，主要从事基因编辑与基因强化育种研究。

创新成果

百合鳞茎发育与休眠机制：对百合鳞片物质代谢进行了空间解析，明确了百合商品种球发育和低温贮藏过程中淀粉、蔗糖代谢的时空模式以及变化规律。从关键酶基因的时空表达角度，初步阐明了百合鳞茎淀粉代谢过程中的蔗糖降解机制。

百合优质种球培育技术：建立了各类百合不定芽诱导、小鳞茎直接发生、愈伤组织间接发生以及体细胞胚发生等不同途径的高效再生和离体快繁技术体系以及埋片繁殖技术体系；建立了百合种球低温贮藏模式以及种球质量评价、检测技术体系。

百合体细胞胚发生机制与应用：构建了体胚发生不同时期miRNA及其靶基因的表达谱，初步构建了百合体胚发生相关miRNA调控网络；建立了基于体细胞胚发生的百合高效遗传转化技术体系，构建了基因编辑技术体系；创制了多种野生百合的多倍体新种质。

百合新品种选育：通过多代杂交，培育出各种株型以及可一年两次产花铁炮百合的新品种；培育出有香气、颜色渐变的岷江百合衍生新品种；创制了一批适于切花、地被、食用的亚洲百合新品种。

百合资源圃  

  百合新品种‘映雪’ 百合团队成员

Shenyang Agricultural University

Lilium Quality Control and Germplasm Innovation Research Team

Team profiles

The research team of lily quality control and germplasm innovation of Shenyang Agricultural University is belong to the key laboratory of Ministry of Education (provincial total construction) for the facilities horticulture, also a member of the National Local Joint Engineering Research Center for the Design and Application Technology of Northern Horticultural facilities. The research team was engaged in lily research for more than 20 years and has undertaken more than 30 research projects at the provincial and ministerial level, of which 8 are funded by the National Natural Science Foundation and 1 by the State key R & D projects. Many awards were obtained including 2 second prize for science and technology progress award of Liaoning province, 2 first prize and 3 second prize for natural science academic achievement of Liaoning province. Fifteen National Invention Patents and 9 Utility Model Patents have been applied for and obtained. More than 200 academic papers, 6 editor-in-chief books and more than 20 collaboratively writed monographs were published. The team now has 2 professors, 1 associate professor, 3 lecturers, 11 doctoral students and 19 master students. Professor Sun Hongmei, team leader, doctoral supervisor, is mainly engaged in lily bulb quality regulation and the mechanism of bulb development and dormancy. Professor Sun Xiaomei, doctoral supervisor, mainly focuses on lily breeding and hybrid breeding. Associate professor Chen Lijing, doctoral supervisor, mainly carries on the research of cold resistance mechanism and germplasm innovation of lily. Lecturer Wang Jinxia dose the research on lily efficient cultivation under facility conditions. Lecturer Wang Chunxia is engaged in the research of lily resource collection and evaluation. Lecturer Wang Zhiping specialize in genome editing technology and gene enhanced breeding.

Innovative results

The mechanism of the development and dormancy of lily bulbs: Spatial analysis of the metabolism of lily scales was carried out. The space-time pattern and the change rule of the starch, the sucrose metabolism during the development and low-temperature storage of the lily bulb were determined. From the time-space expression of the key enzyme genes, the mechanism of the degradation of the sucrose in the process of the starch metabolism of the lily bulbs was preliminarily clarified.

The cultivation technique of superior quality lily bulbs: Different kinds of high efficiency regeneration and in vitro rapid propagation technology systems were established, such as regeneration through adventitious bud induction, direct regeneration in the form of bulblets, indirect regeneration through callus, regeneration through somatic embryogenesis. The low-temperature storage model, the quality evaluation and testing technology systems of lily bulbs were successfully built.

The mechanism and application of somatic embryogenesis in lily: The expression profiles of miRNAs and their target genes in different stages of somatic embryogenesis were constructed, and the miRNA regulatory network related to somatic embryogenesis in lily was preliminarily constructed. An efficient genetic transformation system based on somatic embryogenesis was established and gene editing technique was successfully applied to lily. Many new polyploid germplasm of wild lilies were created.

The selecting and breeding of new variety of lily: New varieties of lily with various plant types and a new variety of Lilium longiflorum which can bloom twice a year were bred by carrying out multi-generation hybridization. A new derived variety of Lilium regale Wilson with fragrance and gradient color was cultivated, also a batch of new Asian lily varieties suitable for cutting flowers, ground flora and edible were created.